Establishment of an animal embryo culture system containing various embryotropins and its efficacy for culturing ICR mouse one-cell embryos derived in vivo or in vitro

Objective: To develop an effective ICR mouse embryo culture medium. Design: In vitro model study. Setting: University-affiliated hospital. Animals: Four-week-old, superovulated mice. Intervention(s): In vivo– or in vitro–derived one-cell embryos were cultured in preimplantation-1 medium (P-1). Main Outcome Measure(s): Preimplantation development. Result(s): In vivo-derived embryos were cultured in BSA-containing P-1, to which one of the following substances was added: [1] no addition, [2] amino acids (aa), [3] aa+hemoglobin (hb), [4] aa+hb+cysteine (cys), [5] aa+hb and glucose (glu) added at the four-cell, or [6] aa+hb and glu+cys added at the four-cell stage. More ( P