Quantitative Determination of Secoiridoid and γ-Pyrone Compounds in Gentiana lutea Cultured in vitro

Abstract The production of secondary metabolites was studied in shoots, roots, and hairy roots of GENTIANA LUTEA obtained IN VITRO. In shoots, both secoiridoid and γ-pyrone compounds were detected in amounts similar to those found in aerial parts of plants collected from nature. The most abundant secoiridoid was gentiopicrin while mangiferin was the main compound among the γ-pyrones. The adventitious roots obtained IN VITRO showed a poor biosynthetic capacity. Upon infection with AGROBACTERIUM RHIZOGENES, nine hairy root clones were established which differed in the amount of secondary metabolites.