Comparison of steady-state trough sirolimus samples by HPLC and a radioreceptor assay
Objectives: We have previously identified a minor immunophilin of 52 kDa molecular weight capable of binding tacrolimus and sirolimus. Because immunophilins are capable of binding both parent drug and metabolites and HPLC assays are typically used to assess parent drug in clinical situations, we use...
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Veröffentlicht in: | Clinical biochemistry 2000-02, Vol.33 (1), p.31-36 |
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Sprache: | eng |
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Zusammenfassung: | Objectives: We have previously identified a minor immunophilin of 52 kDa molecular weight capable of binding tacrolimus and sirolimus. Because immunophilins are capable of binding both parent drug and metabolites and HPLC assays are typically used to assess parent drug in clinical situations, we used this immunophilin in a radioreceptor assay (RRA) to determine if any metabolites not included in the HPLC measurement would bind to the immunophilin and be associated with thrombocytopenia in patients receiving sirolimus.
Design and methods: We tested 51 steady-state trough whole blood samples from non-thrombocytopenic patients and 51 steady-state trough samples from thrombocytopenic patients and compared them to HPLC measurements of parent drug in the same samples. We also tested whole blood samples spiked with authentic sirolimus metabolites using RRA to ascertain the effect these metabolites have on the technique.
Results: We found minimal cross-reactivity in this assay for sirolimus metabolites (binding ranged from |
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ISSN: | 0009-9120 1873-2933 |
DOI: | 10.1016/S0009-9120(99)00088-0 |