Interactions of Cyclodextrins with Dipalmitoyl, Distearoyl, and Dimyristoyl Phosphatidyl Choline Liposomes. A Study by Leakage of Carboxyfluorescein in Inner Aqueous Phase of Unilamellar Liposomes

The interaction of cyclodextrins (CDs) with L-α-dipalmitoyl phopsatidyl choline (DPPC), L-α-distearoyl phosphatidyl choline (DSPC), and L-α-dimyristoyl phosphatidyl choline (DMPC) unilamellar liposomes was investigated by the leakage of carboxylfluorescein (CF) entrapped in the inner aqueous phase of liposomes, at 25°C(DPPC and DSPC liposomes) and at 5°C (DMPC liposomes). The efficiency of CDs for CF leakage was remarkable in the order of heptakis (2, 6-di-O-methyl)-β-CD (DOM-β-CD)>α-CD>heptakis (2, 3, 6-tri-O-methy)-β-CD (TOM-β-CD) from DPPC liposomes, in the order of DOM-β-CD> TOM-β-CO&gt : α-CD from DSPC liposomes and in the order of α-CD>DOM-β-CD