Methodology for optimizing functional miniature proteins based on avian pancreatic polypeptide using phage display

Synthetic genes for avian pancreatic polypeptide (aPP) and for the miniature DNA binding protein PPBR4 were cloned and expressed on the surface of M13 bacteriophage. We anticipate that these constructs will have utility optimizing the properties of miniature proteins based on aPP that result from ou...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Bioorganic & medicinal chemistry letters 2001-06, Vol.11 (12), p.1501-1505
Hauptverfasser:	Chin, Jason W, Grotzfeld, Robert M, Fabian, Miles A, Schepartz, Alanna
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Animals Bacteriophage M13 - metabolism Biological and medical sciences Cloning, Molecular DNA-Binding Proteins - chemical synthesis Drug Design Fundamental and applied biological sciences. Psychology General aspects, investigation methods Methods Pancreatic Polypeptide - chemistry Peptide Fragments - chemistry Peptide Library Protein Structure, Secondary Proteins Structure-Activity Relationship
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	Synthetic genes for avian pancreatic polypeptide (aPP) and for the miniature DNA binding protein PPBR4 were cloned and expressed on the surface of M13 bacteriophage. We anticipate that these constructs will have utility optimizing the properties of miniature proteins based on aPP that result from our previously described protein grafting procedure. A method for optimizing miniature proteins using phage display is reported.
ISSN:	0960-894X 1464-3405
DOI:	10.1016/S0960-894X(01)00139-1