Low frequency vibrations and structural characterization of a murine IgG2a monoclonal antibody studied by Raman and IR spectroscopies

Low frequency vibrations and structural characterization of a murine IgG2a monoclonal antibody studied by Raman and IR spectroscopies

The Raman and ir spectra of a murine IgG2a monoclonal antibody molecule are reported. In accordance with previous studies on immunoglobulins, the secondary structure is predominantly of the β‐sheet type. The low frequency region of the Raman spectrum was also analyzed in detail. A structured band wi...

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Veröffentlicht in:BIOPOLYM 2000-04, Vol.53 (4), p.342-349
Hauptverfasser: Picquart, M., Haro-Poniatowski, E., Morhange, J. F., Jouanne, M., Kanehisa, M.
Format: Artikel
Sprache:eng
Schlagworte:
Animals
Antibodies, Monoclonal - chemistry
Band structure
immunoglobulin
Immunoglobulin G - chemistry
Infrared spectroscopy
ir absorption
low frequency modes
Mice
Mice, Inbred BALB C
Molecular structure
Molecular vibrations
Protein Structure, Secondary
Raman
Raman spectroscopy
Raman, low frequency modes
Spectrophotometry, Infrared - methods
Spectrum Analysis, Raman - methods
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Zusammenfassung:The Raman and ir spectra of a murine IgG2a monoclonal antibody molecule are reported. In accordance with previous studies on immunoglobulins, the secondary structure is predominantly of the β‐sheet type. The low frequency region of the Raman spectrum was also analyzed in detail. A structured band with two maxima near 43 and 94 cm−1 was observed. This band has been attributed to vibrations of elastic body. The results are interpreted using a simple elastic model. © 2000 John Wiley & Sons, Inc. Biopoly 53: 342–349, 2000
ISSN:0006-3525
1097-0282
DOI:10.1002/(SICI)1097-0282(20000405)53:4<342::AID-BIP6>3.0.CO;2-M