AUTOSTAGE SPERM TRACING SYSTEM FOR SEMEN EVALUATION
To overcome the limitation of the microscope field, the study proposed an autostage sperm tracing system (ASTS), which could trace a particular sperm for a long time and distance. The ASTS was constructed by assembling a commercial microscope, an image frame grabber, a personal computer, and a motor...
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| Veröffentlicht in: | Archives of andrology 2000, Vol.44 (1), p.29-39 |
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| creator | KUO, Y. L TZENG, W. L LI, P. C TANG, T. S YOUNG, S. T |
| description | To overcome the limitation of the microscope field, the study proposed an autostage sperm tracing system (ASTS), which could trace a particular sperm for a long time and distance. The ASTS was constructed by assembling a commercial microscope, an image frame grabber, a personal computer, and a motorized stage. Its performance was tested by evaluating 6 semen samples and by comparing the evaluation with those of other semen evaluations. The ASTS broke through the limitation of the microscope field and traced a particular sperm as long as possible. It analyzed the sperm track and calculated the motility parameters, such as curvilinear velocity (Vcl), straight-line velocity (Vsl), and linearity (Lin). The sperm quality was then evaluated in real time, and the user could decide to capture or abandon a particular sperm in the IVF. The ASTS enables users to evaluate sperm progression for a long time and to have the global quality of a particular sperm in real time. Its open structure has the flexibility for micromanipulating a semen sample, and has the potential application associated with a modern IVF technique. |
| doi_str_mv | 10.1080/014850100262380 |
| format | Article |
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L</creatorcontrib><creatorcontrib>TZENG, W. L</creatorcontrib><creatorcontrib>LI, P. C</creatorcontrib><creatorcontrib>TANG, T. S</creatorcontrib><creatorcontrib>YOUNG, S. T</creatorcontrib><title>AUTOSTAGE SPERM TRACING SYSTEM FOR SEMEN EVALUATION</title><title>Archives of andrology</title><addtitle>Arch Androl</addtitle><description>To overcome the limitation of the microscope field, the study proposed an autostage sperm tracing system (ASTS), which could trace a particular sperm for a long time and distance. The ASTS was constructed by assembling a commercial microscope, an image frame grabber, a personal computer, and a motorized stage. Its performance was tested by evaluating 6 semen samples and by comparing the evaluation with those of other semen evaluations. The ASTS broke through the limitation of the microscope field and traced a particular sperm as long as possible. 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Obstetrics</subject><subject>Humans</subject><subject>Image Processing, Computer-Assisted - instrumentation</subject><subject>Image Processing;In Vitro Fertilization;Sperm;Sperm Progression</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Micromanipulation - instrumentation</subject><subject>Sperm Motility - physiology</subject><subject>Spermatozoa - physiology</subject><subject>Sterility. 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Obstetrics</topic><topic>Humans</topic><topic>Image Processing, Computer-Assisted - instrumentation</topic><topic>Image Processing;In Vitro Fertilization;Sperm;Sperm Progression</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Micromanipulation - instrumentation</topic><topic>Sperm Motility - physiology</topic><topic>Spermatozoa - physiology</topic><topic>Sterility. Assisted procreation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>KUO, Y. L</creatorcontrib><creatorcontrib>TZENG, W. L</creatorcontrib><creatorcontrib>LI, P. C</creatorcontrib><creatorcontrib>TANG, T. S</creatorcontrib><creatorcontrib>YOUNG, S. 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T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>AUTOSTAGE SPERM TRACING SYSTEM FOR SEMEN EVALUATION</atitle><jtitle>Archives of andrology</jtitle><addtitle>Arch Androl</addtitle><date>2000</date><risdate>2000</risdate><volume>44</volume><issue>1</issue><spage>29</spage><epage>39</epage><pages>29-39</pages><issn>0955-3002</issn><issn>0148-5016</issn><eissn>1362-3095</eissn><eissn>1521-0375</eissn><coden>ARANDR</coden><abstract>To overcome the limitation of the microscope field, the study proposed an autostage sperm tracing system (ASTS), which could trace a particular sperm for a long time and distance. The ASTS was constructed by assembling a commercial microscope, an image frame grabber, a personal computer, and a motorized stage. Its performance was tested by evaluating 6 semen samples and by comparing the evaluation with those of other semen evaluations. The ASTS broke through the limitation of the microscope field and traced a particular sperm as long as possible. It analyzed the sperm track and calculated the motility parameters, such as curvilinear velocity (Vcl), straight-line velocity (Vsl), and linearity (Lin). The sperm quality was then evaluated in real time, and the user could decide to capture or abandon a particular sperm in the IVF. The ASTS enables users to evaluate sperm progression for a long time and to have the global quality of a particular sperm in real time. Its open structure has the flexibility for micromanipulating a semen sample, and has the potential application associated with a modern IVF technique.</abstract><cop>Philadelphia, PA</cop><pub>Informa UK Ltd</pub><pmid>10690762</pmid><doi>10.1080/014850100262380</doi><tpages>11</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Taylor & Francis Medical Library - CRKN; Access via Taylor & Francis |
| subjects | Algorithms Biological and medical sciences Birth control Fertilization in Vitro - instrumentation Fertilization in Vitro - methods Gynecology. Andrology. Obstetrics Humans Image Processing, Computer-Assisted - instrumentation Image Processing In Vitro Fertilization Sperm Sperm Progression Male Medical sciences Micromanipulation - instrumentation Sperm Motility - physiology Spermatozoa - physiology Sterility. Assisted procreation |
| title | AUTOSTAGE SPERM TRACING SYSTEM FOR SEMEN EVALUATION |
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