AUTOSTAGE SPERM TRACING SYSTEM FOR SEMEN EVALUATION

To overcome the limitation of the microscope field, the study proposed an autostage sperm tracing system (ASTS), which could trace a particular sperm for a long time and distance. The ASTS was constructed by assembling a commercial microscope, an image frame grabber, a personal computer, and a motor...

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Veröffentlicht in:Archives of andrology 2000, Vol.44 (1), p.29-39
Hauptverfasser: KUO, Y. L, TZENG, W. L, LI, P. C, TANG, T. S, YOUNG, S. T
Format: Artikel
Sprache:eng
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Zusammenfassung:To overcome the limitation of the microscope field, the study proposed an autostage sperm tracing system (ASTS), which could trace a particular sperm for a long time and distance. The ASTS was constructed by assembling a commercial microscope, an image frame grabber, a personal computer, and a motorized stage. Its performance was tested by evaluating 6 semen samples and by comparing the evaluation with those of other semen evaluations. The ASTS broke through the limitation of the microscope field and traced a particular sperm as long as possible. It analyzed the sperm track and calculated the motility parameters, such as curvilinear velocity (Vcl), straight-line velocity (Vsl), and linearity (Lin). The sperm quality was then evaluated in real time, and the user could decide to capture or abandon a particular sperm in the IVF. The ASTS enables users to evaluate sperm progression for a long time and to have the global quality of a particular sperm in real time. Its open structure has the flexibility for micromanipulating a semen sample, and has the potential application associated with a modern IVF technique.
ISSN:0955-3002
0148-5016
1362-3095
1521-0375
DOI:10.1080/014850100262380