High throughput on-line solid phase extraction/tandem mass spectrometric determination of paclitaxel in human serum

High throughput on-line solid phase extraction/tandem mass spectrometric determination of paclitaxel in human serum

The feasibility of high throughput on‐line solid phase extraction/tandem mass spectrometry (SPE/MS/MS) is tested for target analysis of paclitaxel in human serum. The use of a dual Prospekt system, with parallel SPE and elution directly to the mass spectrometer, resulted in a cycle time of 80 second...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Rapid communications in mass spectrometry 2000-01, Vol.14 (4), p.230-233
Hauptverfasser: Schellen, Anniek, Ooms, Bert, Gils, Mark van, Halmingh, Otto, Vlis, Erik van der, Lagemaat, Dick van de, Verheij, Elwin
Format: Artikel
Sprache:eng
Schlagworte:
Blood Chemical Analysis - instrumentation
Evaluation Studies as Topic
Humans
Mass Spectrometry - instrumentation
Paclitaxel - blood
Reproducibility of Results
Time Factors
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The feasibility of high throughput on‐line solid phase extraction/tandem mass spectrometry (SPE/MS/MS) is tested for target analysis of paclitaxel in human serum. The use of a dual Prospekt system, with parallel SPE and elution directly to the mass spectrometer, resulted in a cycle time of 80 seconds for the entire, fully automated assay. The assay proved to be linear from 1 to 1000 ng/mL. Cartridges packed with small sorbent particles functioned both as SPE cartridges and as short analytical columns. Copyright © 2000 John Wiley & Sons, Ltd.
ISSN:0951-4198
1097-0231
DOI:10.1002/(SICI)1097-0231(20000229)14:4<230::AID-RCM872>3.0.CO;2-J