Fetal nucleated erythrocyte recovery: Fluorescence activated cell sorting‐based positive selection using anti‐gamma globin versus magnetic activated cell sorting using anti‐CD45 depletion and anti‐gamma globin positive selection

BACKGROUND Fluorescence activated cell sorting (FACS)‐based anti‐gamma (γ) positive selection and magnetic activated cell sorting (MACS)‐based anti‐CD45 depletion followed by anti‐γ positive staining have been two of the most frequently used methods to isolate fetal cells from maternal blood. To dat...

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Veröffentlicht in:	Cytometry (New York, N.Y.) N.Y.), 2000-03, Vol.39 (3), p.224-230
Hauptverfasser:	Wang, Ji Yi, Zhen, Dong Kai, Falco, Vincent M., Farina, Antonio, Zheng, Yuen Ling, Delli‐Bovi, L.C., Bianchi, Diana W.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens, CD - analysis Cell Nucleus - ultrastructure Cell Separation - methods Embryo, Mammalian Erythrocytes - cytology Erythrocytes - ultrastructure Female Fetal Blood - cytology fetal hemoglobin (anti‐γ) fetal nucleated erythrocytes (FNRBCs) Fetus Flow Cytometry - methods fluorescence activated cell sorting (FACS) fluorescence in situ hybridization (FISH) Gestational Age Globins - analysis Humans Immunomagnetic Separation - methods In Situ Hybridization, Fluorescence Leukocyte Common Antigens - analysis magnetic activated cell sorting (MACS) Pregnancy prenatal genetic diagnosis
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