Influence of environmental conditions on dentin bond strengths of recently developed dentin bonding systems

A study was conducted to investigate the influence of temperature and relative humidity (RH) on the bond strengths of several recently developed dentin bonding systems. Six environmental conditions, (A) 25±0.5°C, 50±5% RH, (B) 25±0.5°C, 80±5% RH, (C) 25±0.5°C, 95±5% RH, (D) 37±0.5°C, 50 ±5% RH, (E) 37±0.5°C, 80±5% RH, (F) 37±0.5°C, 95±5% RH were used. Bovine mandibular incisors were mounted in self-curing resin and the facial surfaces were ground on wet #600 SiC paper to expose the dentin. After the tooth surface had been treated according to each manufacturer's instructions, adhesives were applied, followed by condensation of resin composites into a mold placed on the dentin surface. Fifteen specimens per group were stored in distilled water at 37°C for 24 h, and then shear-tested at a cross-head speed of 1.0 mm/min. Statistical analysis was carried out with two-way ANOVA followed by Tukey's test (P