Isolation and Functional Characterization of the Chick M5 Muscarinic Acetylcholine Receptor Gene

: The chick is a widely used system for study of the actions of muscarinic acetylcholine receptors in the cardiovascular, visual, and nervous systems. We report the isolation and functional analysis of the gene encoding the chick M5 muscarinic receptor. RT‐PCR analysis indicates that the M5 receptor...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of neurochemistry 2000-02, Vol.74 (2), p.882-885
Hauptverfasser:	Creason, Sharon, Tietje, Kathleen M., Nathanson, Neil M.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence - genetics Animals Binding, Competitive Biological and medical sciences Brain Brain - embryology Cell receptors Cell structures and functions Chick Chick Embryo - chemistry Chick Embryo - physiology CHO Cells - metabolism CHO Cells - physiology Cricetinae Cyclic AMP Enzyme Activation Fundamental and applied biological sciences. Psychology Gene Expression - physiology Heart - embryology Molecular and cellular biology Molecular Sequence Data Monoamines receptors (catecholamine, serotonine, histamine, acetylcholine) Muscarinic Antagonists - metabolism Muscarinic receptor Phosphoinositides Protein Isoforms - genetics Protein Isoforms - isolation & purification Protein Isoforms - metabolism Protein Isoforms - physiology Receptors, Muscarinic - genetics Receptors, Muscarinic - isolation & purification Receptors, Muscarinic - metabolism Receptors, Muscarinic - physiology Type C Phospholipases - metabolism
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	: The chick is a widely used system for study of the actions of muscarinic acetylcholine receptors in the cardiovascular, visual, and nervous systems. We report the isolation and functional analysis of the gene encoding the chick M5 muscarinic receptor. RT‐PCR analysis indicates that the M5 receptor is expressed at low levels in embryonic chick brain and heart. When expressed in stably transfected Chinese hamster ovary cells, the M5 receptor exhibits high‐affinity binding to muscarinic antagonists and mediates robust activation of phospholipase C activity.
ISSN:	0022-3042 1471-4159
DOI:	10.1046/j.1471-4159.2000.740882.x