Crystallization and preliminary crystallographic data of fructose-1,6-bisphosphatase from human muscle

The enzyme human muscle fructose‐1,6‐bisphosphatase, which plays a critical role in gluconeogenesis, has been crystallized in the presence of 2‐propanol, polyethylene glycol and magnesium chloride at pH 7.5. The space group was determined to be P42212, with unit‐cell parameters a = b = 73.57, c = 14...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2001-06, Vol.57 (6), p.847-849
Hauptverfasser: Zhu, D.-W., Xu, G.-J., Rehse, P. H., Shi, R., Zhao, F.-K., Lin, S.-X.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:The enzyme human muscle fructose‐1,6‐bisphosphatase, which plays a critical role in gluconeogenesis, has been crystallized in the presence of 2‐propanol, polyethylene glycol and magnesium chloride at pH 7.5. The space group was determined to be P42212, with unit‐cell parameters a = b = 73.57, c = 146.50 Å, α = β = λ = 90° and one subunit in the asymmetric unit. A 99.6% complete data set to 2.04 Å has been collected at the National Synchrotron Light Source.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444901004371