Interaction of pea ( Pisum sativum L.) lectins with rhizobial strains

Interaction of pea ( Pisum sativum L.) lectins with rhizobial strains

Lectins from two varieties (PG-3 and LFP-48) of pea have been purified by affinity chromatography on Sephadex G-50. The specific activity increased by 23 and 25 folds, respectively. These lectins from both the varieties were found to be specific for mannose. The purified fluorescein isothiocyanate (...

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Veröffentlicht in:Microbiological research 2001, Vol.156 (1), p.71-74
Hauptverfasser: Bajaj, Manju, Soni, Giridhar, Singh, C.K.
Format: Artikel
Sprache:eng
Schlagworte:
Agronomy. Soil science and plant productions
Biological and medical sciences
Chromatography, Affinity
Economic plant physiology
fluorescein isothiocyanate
Fundamental and applied biological sciences. Psychology
Hemagglutination Inhibition Tests
Lectins - chemistry
Lectins - isolation & purification
Lectins - metabolism
Lectins Pea
mannose
Mannose - physiology
Microscopy, Fluorescence
Pisum sativum
Pisum sativum - chemistry
Pisum sativum - metabolism
Pisum sativum - microbiology
Pisum sativum L.
Plant Lectins
Rhizobium
Rhizobium - chemistry
Rhizobium - growth & development
Rhizobium - metabolism
Symbiosis (nodules, symbiotic nitrogen fixation, mycorrhiza...)
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Zusammenfassung:Lectins from two varieties (PG-3 and LFP-48) of pea have been purified by affinity chromatography on Sephadex G-50. The specific activity increased by 23 and 25 folds, respectively. These lectins from both the varieties were found to be specific for mannose. The purified fluorescein isothiocyanate (FITC) – labelled lectins showed binding reaction with homologous as well as heterologous strains of Rhizobium spp. The results revealed that pea lectins are not highly specific to their respective rhizobia. Moreover, these lectins showed a greater stimulatory effect on homologous Rhizobium leguminosarum strains.
ISSN:0944-5013
1618-0623
DOI:10.1078/0944-5013-00080