A novel method for detecting IgA endomysial antibodies by using human umbilical vein endothelial cells

BACKGROUND OBJECTIVES SETTING PATIENTS AND METHODS RESULTS CONCLUSIONS(1) As a substrate for anti-endomysial antibody, HUVEC may provide the same diagnostic accuracy as monkey oesophagus, thus bypassing economical and ethical problems. The HUVEC antigen reacting with IgA from coeliac disease sera is...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	European journal of gastroenterology & hepatology 2000-01, Vol.12 (1), p.45-49
Hauptverfasser:	Castellino, Francesca, Scaglione, Nadia, Grosso, Silvia B, Sategna-Guidetti, Carla
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Animals Autoantibodies - blood Autoantibodies - isolation & purification Autoantigens - immunology Biological and medical sciences Case-Control Studies Celiac Disease - blood Celiac Disease - diagnosis Celiac Disease - immunology Cell Line Digestive system Endothelium, Vascular - cytology Endothelium, Vascular - enzymology Endothelium, Vascular - immunology Esophagus - cytology Female Fluorescent Antibody Technique, Indirect Haplorhini Humans Immunoglobulin A - isolation & purification Investigative techniques, diagnostic techniques (general aspects) Male Medical sciences Muscle, Smooth, Vascular - enzymology Muscle, Smooth, Vascular - immunology Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Transglutaminases - immunology Umbilical Veins - cytology Umbilical Veins - enzymology Umbilical Veins - immunology
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	BACKGROUND OBJECTIVES SETTING PATIENTS AND METHODS RESULTS CONCLUSIONS(1) As a substrate for anti-endomysial antibody, HUVEC may provide the same diagnostic accuracy as monkey oesophagus, thus bypassing economical and ethical problems. The HUVEC antigen reacting with IgA from coeliac disease sera is an intracellular rather than a cell-surface antigen, as IgA antibodies reacted only with permeabilized cells. (2) Pretreatment of untreated coeliac sera with tissue transglutaminase abolished almost completely the specific staining; incubation with anti-tissue transglutaminase antibody elicited the characteristic fluorescent pattern, thus confirming that tissue transglutaminase represents the prominent autoantigen in coeliac disease. Eur J Gastroenterol Hepatol 12:45-49 © 2000 Lippincott Williams & WilkinsEuropean Journal of Gastroenterology & Hepatology 2000, 12:45-49
ISSN:	0954-691X 1473-5687
DOI:	10.1097/00042737-200012010-00009