Analysis of the Key pfcrt Point Mutation and In Vitro and In Vivo Response to Chloroquine in Yaoundé, Cameroon

The putative key codon (Lys-76 in sensitive parasites and Thr-76 in resistant parasites) of the novel candidate gene for chloroquine resistance, Plasmodium falciparum chloroquine resistance transporter (pfcrt) was determined by polymerase chain reaction–restriction fragment length polymorphism from 111 Cameroonian isolates and was compared with in vivo and in vitro responses to chloroquine. The key codon was significantly associated (P