Protection of MLV Vector Particles from Human Complement

Murine cell-derived MLV vector particles usually are highly sensitive to human complement-mediated lysis. Expression of the human complement inhibitor CD59 on murine packaging cells resulted in partial protection of these cells from lysis caused by human complement proteins. Furthermore, CD59 was in...

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Veröffentlicht in:	Biochemical and biophysical research communications 1999-10, Vol.264 (1), p.1-5
Hauptverfasser:	Breun, Sabine, Salmons, Brian, Günzburg, Walter H., Baumann, Jörg G.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals CD59 Antigens - genetics CD59 Antigens - immunology Cells, Cultured Complement Inactivator Proteins - genetics Complement Inactivator Proteins - immunology Complement System Proteins - immunology Complement System Proteins - metabolism Genetic Vectors - genetics Humans Leukemia Virus, Murine - genetics Leukemia Virus, Murine - immunology Mice Murine leukemia virus Transfection
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Beschreibung
Zusammenfassung:	Murine cell-derived MLV vector particles usually are highly sensitive to human complement-mediated lysis. Expression of the human complement inhibitor CD59 on murine packaging cells resulted in partial protection of these cells from lysis caused by human complement proteins. Furthermore, CD59 was incorporated into MLV vector particles released by these packaging cells, leading to an improved resistance of the virions against human complement-mediated inactivation.
ISSN:	0006-291X 1090-2104
DOI:	10.1006/bbrc.1999.1474