Histochemical localization of reverse transcriptase in polytene chromosomes of chironomids
The localization of a reverse transcriptase-related protein in salivary gland polytene chromosomes was investigated by immunohistochemistry in two species of Chironomus. The antibodies used were raised against a recombinant protein containing phylogenetically conserved motifs of reverse transcriptas...
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Veröffentlicht in: | Chromosoma 1999-09, Vol.108 (5), p.302-307 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | The localization of a reverse transcriptase-related protein in salivary gland polytene chromosomes was investigated by immunohistochemistry in two species of Chironomus. The antibodies used were raised against a recombinant protein containing phylogenetically conserved motifs of reverse transcriptases and derived from an abundant non-LTR element previously identified in Chironomus. Immunoreactive protein was found in some telomeres, in a centromeric region, in a few interstitial bands and in Balbiani ring 3. The telomeric signal was probably dependent on transcription and increased dramatically when telomeric heat shock puffs were induced. A correlation with transcription was also seen in Balbiani ring 3, the immunobinding of which disappeared after inhibition of transcription with actinomycin D. |
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ISSN: | 0009-5915 1432-0886 |
DOI: | 10.1007/s004120050381 |