Transverse-Relaxation-Optimized (TROSY) Gradient-Enhanced Triple-Resonance NMR Spectroscopy
Two modifications to sensitivity-enhanced gradient-selected TROSY-based triple-resonance NMR experiments are proposed that reduce the overall duration of the pulse sequences and minimize radiation damping effects on water-flipback solvent suppression. The modifications are illustrated for the HNCO–T...
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| Veröffentlicht in: | Journal of magnetic resonance (1997) 1999-11, Vol.141 (1), p.180-184 |
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| container_end_page | 184 |
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| container_title | Journal of magnetic resonance (1997) |
| container_volume | 141 |
| creator | Loria, J.Patrick Rance, Mark Palmer, Arthur G. |
| description | Two modifications to sensitivity-enhanced gradient-selected TROSY-based triple-resonance NMR experiments are proposed that reduce the overall duration of the pulse sequences and minimize radiation damping effects on water-flipback solvent suppression. The modifications are illustrated for the HNCO–TROSY experiment, but are applicable to all triple-resonance experiments that detect proton magnetization after a reverse polarization transfer step from a 15N spin. The methods are applied to yeast triosephosphate isomerase, a symmetric dimer with 248 amino acid residues per monomer. |
| doi_str_mv | 10.1006/jmre.1999.1891 |
| format | Article |
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| ispartof | Journal of magnetic resonance (1997), 1999-11, Vol.141 (1), p.180-184 |
| issn | 1090-7807 1096-0856 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | gradient coherence selection Magnetic Resonance Spectroscopy - methods triosephosphate isomerase triple-resonance NMR spectroscopy TROSY |
| title | Transverse-Relaxation-Optimized (TROSY) Gradient-Enhanced Triple-Resonance NMR Spectroscopy |
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