Transverse-Relaxation-Optimized (TROSY) Gradient-Enhanced Triple-Resonance NMR Spectroscopy

Two modifications to sensitivity-enhanced gradient-selected TROSY-based triple-resonance NMR experiments are proposed that reduce the overall duration of the pulse sequences and minimize radiation damping effects on water-flipback solvent suppression. The modifications are illustrated for the HNCO–T...

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Veröffentlicht in:	Journal of magnetic resonance (1997) 1999-11, Vol.141 (1), p.180-184
Hauptverfasser:	Loria, J.Patrick, Rance, Mark, Palmer, Arthur G.
Format:	Artikel
Sprache:	eng
Schlagworte:	gradient coherence selection Magnetic Resonance Spectroscopy - methods triosephosphate isomerase triple-resonance NMR spectroscopy TROSY
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Beschreibung
Zusammenfassung:	Two modifications to sensitivity-enhanced gradient-selected TROSY-based triple-resonance NMR experiments are proposed that reduce the overall duration of the pulse sequences and minimize radiation damping effects on water-flipback solvent suppression. The modifications are illustrated for the HNCO–TROSY experiment, but are applicable to all triple-resonance experiments that detect proton magnetization after a reverse polarization transfer step from a 15N spin. The methods are applied to yeast triosephosphate isomerase, a symmetric dimer with 248 amino acid residues per monomer.
ISSN:	1090-7807 1096-0856
DOI:	10.1006/jmre.1999.1891