Hydrogen Peroxide Mediates the Cell Growth and Transformation Caused by the Mitogenic Oxidase Nox1
Nox1, a homologue of gp91phox, the catalytic moiety of the superoxide (O2-)-generating NADPH oxidase of phagocytes, causes increased O2- generation, increased mitotic rate, cell transformation, and tumorigenicity when expressed in NIH 3T3 fibroblasts. This study explores the role of reactive oxygen...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2001-05, Vol.98 (10), p.5550-5555 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Arnold, Rebecca S. Shi, Jing Murad, Emma Whalen, Anne M. Sun, Carrie Q. Polavarapu, Rathnagiri Parthasarathy, Sampath Petros, John A. Lambeth, J. David |
| description | Nox1, a homologue of gp91phox, the catalytic moiety of the superoxide (O2-)-generating NADPH oxidase of phagocytes, causes increased O2- generation, increased mitotic rate, cell transformation, and tumorigenicity when expressed in NIH 3T3 fibroblasts. This study explores the role of reactive oxygen species (ROS) in regulating cell growth and transformation by Nox1. H2O2 concentration increased ≈10-fold in Nox1-expressing cells, compared with |
| doi_str_mv | 10.1073/pnas.101505898 |
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David</creator><creatorcontrib>Arnold, Rebecca S. ; Shi, Jing ; Murad, Emma ; Whalen, Anne M. ; Sun, Carrie Q. ; Polavarapu, Rathnagiri ; Parthasarathy, Sampath ; Petros, John A. ; Lambeth, J. David</creatorcontrib><description>Nox1, a homologue of gp91phox, the catalytic moiety of the superoxide (O2-)-generating NADPH oxidase of phagocytes, causes increased O2- generation, increased mitotic rate, cell transformation, and tumorigenicity when expressed in NIH 3T3 fibroblasts. This study explores the role of reactive oxygen species (ROS) in regulating cell growth and transformation by Nox1. H2O2 concentration increased ≈10-fold in Nox1-expressing cells, compared with <2-fold increase in O2-. When human catalase was expressed in Nox1-expressing cells, H2O2 concentration decreased, and the cells reverted to a normal appearance, the growth rate normalized, and cells no longer produced tumors in athymic mice. A large number of genes, including many related to cell cycle, growth, and cancer (but unrelated to oxidative stress), were expressed in Nox1-expressing cells, and more than 60% of these returned to normal levels on coexpression of catalase. Thus, H2O2 in low concentrations functions as an intracellular signal that triggers a genetic program related to cell growth.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.101505898</identifier><identifier>PMID: 11331784</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>3T3 cells ; Animals ; Base Sequence ; Biochemistry ; Biological Sciences ; Catalase - metabolism ; Cell cycle ; Cell Division - drug effects ; Cell Division - physiology ; Cell growth ; Cell Line ; Cell lines ; Cell Transformation, Neoplastic - drug effects ; Cells ; DNA Primers ; Enzymes ; Fluorescence ; Hydrogen Peroxide - pharmacology ; Mice ; Mice, Nude ; NADH, NADPH Oxidoreductases - physiology ; NADPH Oxidase 1 ; NIH 3T3 cells ; Nox1 gene ; Phenotypes ; Physical growth ; Reactive oxygen species ; Reverse Transcriptase Polymerase Chain Reaction ; Tumor cell line ; Tumors</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2001-05, Vol.98 (10), p.5550-5555</ispartof><rights>Copyright 1993-2001 National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences May 8, 2001</rights><rights>Copyright © 2001, The National Academy of Sciences 2001</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c584t-4e452848f6997c243299997a88ec4336352efa4d1b7548306dcf8b6beabcee3</citedby><cites>FETCH-LOGICAL-c584t-4e452848f6997c243299997a88ec4336352efa4d1b7548306dcf8b6beabcee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/98/10.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3055664$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3055664$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/11331784$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Arnold, Rebecca S.</creatorcontrib><creatorcontrib>Shi, Jing</creatorcontrib><creatorcontrib>Murad, Emma</creatorcontrib><creatorcontrib>Whalen, Anne M.</creatorcontrib><creatorcontrib>Sun, Carrie Q.</creatorcontrib><creatorcontrib>Polavarapu, Rathnagiri</creatorcontrib><creatorcontrib>Parthasarathy, Sampath</creatorcontrib><creatorcontrib>Petros, John A.</creatorcontrib><creatorcontrib>Lambeth, J. David</creatorcontrib><title>Hydrogen Peroxide Mediates the Cell Growth and Transformation Caused by the Mitogenic Oxidase Nox1</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Nox1, a homologue of gp91phox, the catalytic moiety of the superoxide (O2-)-generating NADPH oxidase of phagocytes, causes increased O2- generation, increased mitotic rate, cell transformation, and tumorigenicity when expressed in NIH 3T3 fibroblasts. This study explores the role of reactive oxygen species (ROS) in regulating cell growth and transformation by Nox1. H2O2 concentration increased ≈10-fold in Nox1-expressing cells, compared with <2-fold increase in O2-. When human catalase was expressed in Nox1-expressing cells, H2O2 concentration decreased, and the cells reverted to a normal appearance, the growth rate normalized, and cells no longer produced tumors in athymic mice. A large number of genes, including many related to cell cycle, growth, and cancer (but unrelated to oxidative stress), were expressed in Nox1-expressing cells, and more than 60% of these returned to normal levels on coexpression of catalase. Thus, H2O2 in low concentrations functions as an intracellular signal that triggers a genetic program related to cell growth.</description><subject>3T3 cells</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biochemistry</subject><subject>Biological Sciences</subject><subject>Catalase - metabolism</subject><subject>Cell cycle</subject><subject>Cell Division - drug effects</subject><subject>Cell Division - physiology</subject><subject>Cell growth</subject><subject>Cell Line</subject><subject>Cell lines</subject><subject>Cell Transformation, Neoplastic - drug effects</subject><subject>Cells</subject><subject>DNA Primers</subject><subject>Enzymes</subject><subject>Fluorescence</subject><subject>Hydrogen Peroxide - pharmacology</subject><subject>Mice</subject><subject>Mice, Nude</subject><subject>NADH, NADPH Oxidoreductases - physiology</subject><subject>NADPH Oxidase 1</subject><subject>NIH 3T3 cells</subject><subject>Nox1 gene</subject><subject>Phenotypes</subject><subject>Physical growth</subject><subject>Reactive oxygen species</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Tumor cell line</subject><subject>Tumors</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFvFCEUxonR2O3q1ZMxxEM9TYUBZiDxYjbamrTWxN4Jw7zpspkdVmB097-X6a5r9aAkBJL3-z7e40PoBSXnlNTs7WYwMd-oIEIq-QjNKFG0qLgij9GMkLIuJC_5CTqNcUUIUUKSp-iEUsZoLfkMNZe7Nvg7GPAXCH7rWsDX0DqTIOK0BLyAvscXwf9IS2yGFt8GM8TOh7VJzg94YcYILW529_C1S5OVs_gmO5kI-LPf0mfoSWf6CM8P5xx9_fjhdnFZXN1cfFq8vyqskDwVHLgoJZddpVRtS85KlVdtpATLGauYKKEzvKVNLbhkpGptJ5uqAdNYADZH7_aum7FZQ2thSMH0ehPc2oSd9sbpPyuDW-o7_10zVgqS5WcHefDfRohJr120eXgzgB-jronk-c3_g_lbc6t5z9Hrv8CVH8OQf0CXhLJacaUydL6HbPAxBuiODVOip4D1FLA-BpwFrx6O-Rs_JPoAmIS_ykpOhkLcT_rmn4Duxr5PsE2ZfLknVzH5cEQZEaKqOPsJvrbDVA</recordid><startdate>20010508</startdate><enddate>20010508</enddate><creator>Arnold, Rebecca S.</creator><creator>Shi, Jing</creator><creator>Murad, Emma</creator><creator>Whalen, Anne M.</creator><creator>Sun, Carrie Q.</creator><creator>Polavarapu, Rathnagiri</creator><creator>Parthasarathy, Sampath</creator><creator>Petros, John A.</creator><creator>Lambeth, J. David</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><general>The National Academy of Sciences</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20010508</creationdate><title>Hydrogen Peroxide Mediates the Cell Growth and Transformation Caused by the Mitogenic Oxidase Nox1</title><author>Arnold, Rebecca S. ; Shi, Jing ; Murad, Emma ; Whalen, Anne M. ; Sun, Carrie Q. ; Polavarapu, Rathnagiri ; Parthasarathy, Sampath ; Petros, John A. ; Lambeth, J. 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When human catalase was expressed in Nox1-expressing cells, H2O2 concentration decreased, and the cells reverted to a normal appearance, the growth rate normalized, and cells no longer produced tumors in athymic mice. A large number of genes, including many related to cell cycle, growth, and cancer (but unrelated to oxidative stress), were expressed in Nox1-expressing cells, and more than 60% of these returned to normal levels on coexpression of catalase. Thus, H2O2 in low concentrations functions as an intracellular signal that triggers a genetic program related to cell growth.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>11331784</pmid><doi>10.1073/pnas.101505898</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 3T3 cells Animals Base Sequence Biochemistry Biological Sciences Catalase - metabolism Cell cycle Cell Division - drug effects Cell Division - physiology Cell growth Cell Line Cell lines Cell Transformation, Neoplastic - drug effects Cells DNA Primers Enzymes Fluorescence Hydrogen Peroxide - pharmacology Mice Mice, Nude NADH, NADPH Oxidoreductases - physiology NADPH Oxidase 1 NIH 3T3 cells Nox1 gene Phenotypes Physical growth Reactive oxygen species Reverse Transcriptase Polymerase Chain Reaction Tumor cell line Tumors |
| title | Hydrogen Peroxide Mediates the Cell Growth and Transformation Caused by the Mitogenic Oxidase Nox1 |
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