Hydrogen Peroxide Mediates the Cell Growth and Transformation Caused by the Mitogenic Oxidase Nox1

Nox1, a homologue of gp91phox, the catalytic moiety of the superoxide (O2-)-generating NADPH oxidase of phagocytes, causes increased O2- generation, increased mitotic rate, cell transformation, and tumorigenicity when expressed in NIH 3T3 fibroblasts. This study explores the role of reactive oxygen...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 2001-05, Vol.98 (10), p.5550-5555
Hauptverfasser:	Arnold, Rebecca S., Shi, Jing, Murad, Emma, Whalen, Anne M., Sun, Carrie Q., Polavarapu, Rathnagiri, Parthasarathy, Sampath, Petros, John A., Lambeth, J. David
Format:	Artikel
Sprache:	eng
Schlagworte:	3T3 cells Animals Base Sequence Biochemistry Biological Sciences Catalase - metabolism Cell cycle Cell Division - drug effects Cell Division - physiology Cell growth Cell Line Cell lines Cell Transformation, Neoplastic - drug effects Cells DNA Primers Enzymes Fluorescence Hydrogen Peroxide - pharmacology Mice Mice, Nude NADH, NADPH Oxidoreductases - physiology NADPH Oxidase 1 NIH 3T3 cells Nox1 gene Phenotypes Physical growth Reactive oxygen species Reverse Transcriptase Polymerase Chain Reaction Tumor cell line Tumors
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Zusammenfassung:	Nox1, a homologue of gp91phox, the catalytic moiety of the superoxide (O2-)-generating NADPH oxidase of phagocytes, causes increased O2- generation, increased mitotic rate, cell transformation, and tumorigenicity when expressed in NIH 3T3 fibroblasts. This study explores the role of reactive oxygen species (ROS) in regulating cell growth and transformation by Nox1. H2O2 concentration increased ≈10-fold in Nox1-expressing cells, compared with
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.101505898