Effect of Sealing and Tween 80 on the Antifungal Susceptibility Testing of Essential Oils

Concentrations of essential oils showing high volatility decreased substantially in broth and agar media when incubated under open conditions. The decrease in the half life was from 0.7 to 38 hr in broth medium at 27 C. When evaporation was prevented by sealing, MIC values against Aspergillus fumiga...

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Veröffentlicht in:	Microbiology and immunology 2001-01, Vol.45 (3), p.201-208
Hauptverfasser:	Inouye, Shigeharu, Tsuruoka, Tsutomu, Uchida, Katsuhisa, Yamaguchi, Hideyo
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibiotics. Antiinfectious agents. Antiparasitic agents Antifungal agents Antifungal Agents - pharmacology antifungal test Aspergillus fumigatus Aspergillus fumigatus - drug effects Biological and medical sciences Candida albicans Candida albicans - drug effects Culture Media essential oils Medical sciences Microbial Sensitivity Tests - methods Mitosporic Fungi - drug effects Oils, Volatile - pharmacology Pharmacology. Drug treatments Polysorbates sealing sealing, tween 80 Time Factors Trichophyton - drug effects Tween 80
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Zusammenfassung:	Concentrations of essential oils showing high volatility decreased substantially in broth and agar media when incubated under open conditions. The decrease in the half life was from 0.7 to 38 hr in broth medium at 27 C. When evaporation was prevented by sealing, MIC values against Aspergillus fumigatus, Candida albicans and Trichophyton mentagrophytes by broth or agar dilution assay were lowered two to eight‐fold, as compared with those obtained under open conditions. Addition of Tween 80 caused a rise of the MICs against A. fumigatus by two to four‐fold in broth dilution assay, but little affected the MICs in agar dilution assay.
ISSN:	0385-5600 1348-0421
DOI:	10.1111/j.1348-0421.2001.tb02608.x