Rat retinal progenitor cells and a retinal pigment epithelial factor

Retinal progenitor cells were isolated from explants of neonatal rat retinas and characterized by transmission electron microscopy and reverse transcriptase-polymerase chain reaction and by their response to an isolated retinal pigment epithelial cell cell factor. The isolated progenitor cells demon...

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Veröffentlicht in:	Brain research. Developmental brain research 2001-04, Vol.127 (2), p.185-187
Hauptverfasser:	Sheedlo, Harold J, Brun-Zinkernagel, Anne-Maire, Oakford, Larry X, Roque, Rouel S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Survival Cells, Cultured Intermediate Filament Proteins - genetics Microscopy, Electron Nerve Tissue Proteins Nestin Opsin Pigment Epithelium of Eye - chemistry Progenitor cell Rats Rats, Long-Evans Retina Retina - cytology Retinal pigment epithelium Reverse transcriptase-polymerase chain reaction RNA, Messenger - analysis Rod Opsins - genetics Stem Cells - physiology Stem Cells - ultrastructure
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Zusammenfassung:	Retinal progenitor cells were isolated from explants of neonatal rat retinas and characterized by transmission electron microscopy and reverse transcriptase-polymerase chain reaction and by their response to an isolated retinal pigment epithelial cell cell factor. The isolated progenitor cells demonstrated nuclei with abundant euchromatin typical of progenitor cells and showed the presence of nestin and opsin message. A protein (∼67 kDa) isolated from conditioned media of cultured rat RPE cells promoted the survival of isolated retinal progenitor cells.
ISSN:	0165-3806
DOI:	10.1016/S0165-3806(01)00118-3