A multicopper oxidase gene from Candida albicans: cloning, characterization and disruption

Hans-Knöll-Institut für Naturstoff-Forschung eV, Abteilung Mikrobielle Infektionsbiologie, Beutenbergstrasse 11,D-07745 Jena, Germany 1 Author for correspondence: Raimund Eck. Tel: +49 3641 656852. Fax: +49 3641 656652. e-mail: reck{at}pmail.hki-jena.de A multicopper oxidase gene from the human path...

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Veröffentlicht in:Microbiology (Society for General Microbiology) 1999-09, Vol.145 (9), p.2415-2422
Hauptverfasser: Eck, Raimund, Hundt, Susanne, Hartl, Albert, Roemer, Ernst, Kunkel, Waldemar
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Sprache:eng
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Zusammenfassung:Hans-Knöll-Institut für Naturstoff-Forschung eV, Abteilung Mikrobielle Infektionsbiologie, Beutenbergstrasse 11,D-07745 Jena, Germany 1 Author for correspondence: Raimund Eck. Tel: +49 3641 656852. Fax: +49 3641 656652. e-mail: reck{at}pmail.hki-jena.de A multicopper oxidase gene from the human pathogenic yeast Candida albicans was isolated and characterized. An open reading frame of 1872 bp, designated CaFET3 , was identified, encoding a predicted protein of 624 amino acids and a molecular mass of 70·5 kDa. The identity between the deduced amino acid sequences of CaFET3 and the Saccharomyces cerevisiae FET3 gene is 55%. CaFET3 was localized on chromosome 6. A null mutant ( fet3 / fet3 ) was constructed by sequential gene disruption. Unlike the C. albicans SC5314 wild-type strain the fet3 mutant was unable to grow in low-iron medium. The lack of growth of a S. cerevisiae fet3 mutant in iron-limited medium was compensated by transformation with CaFET3 . The null mutant strain showed no change in pathogenicity compared with the wild-type strain in the mouse model of systemic candidiasis. Keywords: multicopper oxidase, Candida albicans , gene disruption, pathogenicity, iron uptake Abbreviations: BPA, bathophenanthrolinedisulfonic acid b The EMBL accession number for the sequence reported in this paper is Y09329.
ISSN:1350-0872
1465-2080
DOI:10.1099/00221287-145-9-2415