Biosynthesis of poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) by metabolically engineered Escherichia coli strains

The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3‐hydroxybutyrate (3HB), 3‐hydroxyvalerate (3HV), and 3‐hydroxyhexanoate (3HHx) [P(3HB‐co‐3HV‐co‐3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In a...

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Veröffentlicht in:	Biotechnology and bioengineering 2001-07, Vol.74 (1), p.82-87
Hauptverfasser:	Park, Si Jae, Ahn, Woo Suk, Green, Phillip R., Lee, Sang Yup
Format:	Artikel
Sprache:	eng
Schlagworte:	3-hydroxybutyric acid 3-hydroxyhexanoic acid 3-hydroxyvaleric acid Acyltransferases - genetics Acyltransferases - metabolism Aeromonas - genetics Aeromonas hydrophila b-Hydroxybutyric acid Biological and medical sciences Biotechnology dodecanoic acid Enoyl-CoA Hydratase - genetics Enoyl-CoA Hydratase - metabolism Escherichia coli Escherichia coli - genetics Escherichia coli - metabolism Fermentation Fundamental and applied biological sciences. Psychology Genetic Engineering Lauric Acids - metabolism Methods. Procedures. Technologies Microbial engineering. Fermentation and microbial culture technology orf1 gene poly(3-hydroxybutyrate-co-3-hydroxyvalerate-co-3-hydroxyhexanoate) polyhydroxyalkanoates Polymers - metabolism terpolyester
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Zusammenfassung:	The recombinant Escherichia coli strain, equipped with the newly cloned Aeromonas PHA biosynthesis genes, could produce a terpolymer of 3‐hydroxybutyrate (3HB), 3‐hydroxyvalerate (3HV), and 3‐hydroxyhexanoate (3HHx) [P(3HB‐co‐3HV‐co‐3HHx)] from dodecanoic acid plus odd carbon number fatty acid. In addition, the orf1 gene of Aeromonas hydrophila was found to play a critical role in assimilating the 3HV monomer and in regulating the monomer fraction in the terpolymer. © 2001 John Wiley & Sons, Inc. Biotechnol Bioeng 74: 81–86, 2001.
ISSN:	0006-3592 1097-0290
DOI:	10.1002/bit.1097