Identification and characterization of a cystathionine β/γ-lyase from Lactococcus lactis ssp. cremoris MG1363
This paper describes the nucleotide sequence of a gene encoding cystathionine β/γ-lyase from Lactococcus lactis ssp. cremoris MG1363, its overexpression in Escherichia coli and some functional characteristics of the purified recombinant protein.
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| Veröffentlicht in: | FEMS microbiology letters 2000-01, Vol.182 (2), p.249-254 |
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| container_end_page | 254 |
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| container_issue | 2 |
| container_start_page | 249 |
| container_title | FEMS microbiology letters |
| container_volume | 182 |
| creator | Dobric, Nada Limsowtin, Gäetan K.Y. Hillier, Alan J. Dudman, Nicholas P.B. Davidson, Barrie E. |
| description | This paper describes the nucleotide sequence of a gene encoding cystathionine β/γ-lyase from
Lactococcus lactis ssp.
cremoris MG1363, its overexpression in
Escherichia coli and some functional characteristics of the purified recombinant protein. |
| doi_str_mv | 10.1016/S0378-1097(99)00599-6 |
| format | Article |
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Lactococcus lactis ssp.
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Lactococcus lactis ssp.
cremoris MG1363, its overexpression in
Escherichia coli and some functional characteristics of the purified recombinant protein.</description><subject>Amino acid derivatization</subject><subject>Amino Acid Sequence</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cheese - microbiology</subject><subject>Cheese flavor</subject><subject>Chromatography, Affinity</subject><subject>Chromatography, High Pressure Liquid</subject><subject>cystathionine</subject><subject>Cystathionine gamma-Lyase - chemistry</subject><subject>Cystathionine gamma-Lyase - genetics</subject><subject>Cystathionine gamma-Lyase - isolation & purification</subject><subject>Cystathionine gamma-Lyase - metabolism</subject><subject>Cystathionine lyase</subject><subject>Cysteine - metabolism</subject><subject>Escherichia coli</subject><subject>functional properties</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene overexpression</subject><subject>Genes, Bacterial</subject><subject>Homocysteine - metabolism</subject><subject>Lactic acid bacterium</subject><subject>Lactococcus lactis - enzymology</subject><subject>Lactococcus lactis - genetics</subject><subject>Lactococcus lactis subsp. cremoris</subject><subject>Metabolism. Enzymes</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequences</subject><subject>recombinant proteins</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Sequence Alignment</subject><subject>Sequence Analysis, DNA</subject><subject>Substrate Specificity</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcFu1DAQhi0EokvhEQAfECqHtHa8seMTQlVpKy3iUHq2nPGYGiXxYmeRto_VvkefCW-zAm6cPGN__8z4H0Jec3bMGZcnV0yotuJMqyOtPzDWaF3JJ2TBG7WspJbtU7L4gxyQFzn_YIwtayafkwPOZAnUckHipcNxCj6AnUIcqR0dhRubLEyYwu18GT21FLZ5stNNycOI9OHu5OG-6rc2I_UpDnRVFBEiwCbTvsQh05zXxxQSDjGV7Ms5F1K8JM-87TO-2p-H5Prz2bfTi2r19fzy9NOqglqpqbLCOYeiazXXwnvddsAUAvO2fAfBcqHrRtfMN6ypG-VEo1nXiRbLg1O8Fofk_Vx3neLPDebJDCED9r0dMW6yUazlRbMDmxmEFHNO6M06hcGmreHM7Jw2j06bnY1Ga_PotJFF92bfYNMN6P5RzdYW4N0esBls75MdIeS_XC3qsqaCvZ0xb6Ox34tT5vqqLn3LtrRcyt2EH2cCi1-_AiaTIeAI6EJCmIyL4T-z_gbHgaVQ</recordid><startdate>20000115</startdate><enddate>20000115</enddate><creator>Dobric, Nada</creator><creator>Limsowtin, Gäetan K.Y.</creator><creator>Hillier, Alan J.</creator><creator>Dudman, Nicholas P.B.</creator><creator>Davidson, Barrie E.</creator><general>Elsevier B.V</general><general>Blackwell</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20000115</creationdate><title>Identification and characterization of a cystathionine β/γ-lyase from Lactococcus lactis ssp. cremoris MG1363</title><author>Dobric, Nada ; Limsowtin, Gäetan K.Y. ; Hillier, Alan J. ; Dudman, Nicholas P.B. ; Davidson, Barrie E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c277t-a3ddde3b89193ff98bc07ec0fa109eca13925920f505257d3590bb38e139d7123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Amino acid derivatization</topic><topic>Amino Acid Sequence</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cheese - microbiology</topic><topic>Cheese flavor</topic><topic>Chromatography, Affinity</topic><topic>Chromatography, High Pressure Liquid</topic><topic>cystathionine</topic><topic>Cystathionine gamma-Lyase - chemistry</topic><topic>Cystathionine gamma-Lyase - genetics</topic><topic>Cystathionine gamma-Lyase - isolation & purification</topic><topic>Cystathionine gamma-Lyase - metabolism</topic><topic>Cystathionine lyase</topic><topic>Cysteine - metabolism</topic><topic>Escherichia coli</topic><topic>functional properties</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene overexpression</topic><topic>Genes, Bacterial</topic><topic>Homocysteine - metabolism</topic><topic>Lactic acid bacterium</topic><topic>Lactococcus lactis - enzymology</topic><topic>Lactococcus lactis - genetics</topic><topic>Lactococcus lactis subsp. cremoris</topic><topic>Metabolism. 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| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2000-01, Vol.182 (2), p.249-254 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Amino acid derivatization Amino Acid Sequence Bacteriology Biological and medical sciences Cheese - microbiology Cheese flavor Chromatography, Affinity Chromatography, High Pressure Liquid cystathionine Cystathionine gamma-Lyase - chemistry Cystathionine gamma-Lyase - genetics Cystathionine gamma-Lyase - isolation & purification Cystathionine gamma-Lyase - metabolism Cystathionine lyase Cysteine - metabolism Escherichia coli functional properties Fundamental and applied biological sciences. Psychology gene overexpression Genes, Bacterial Homocysteine - metabolism Lactic acid bacterium Lactococcus lactis - enzymology Lactococcus lactis - genetics Lactococcus lactis subsp. cremoris Metabolism. Enzymes Microbiology Molecular Sequence Data nucleotide sequences recombinant proteins Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Alignment Sequence Analysis, DNA Substrate Specificity |
| title | Identification and characterization of a cystathionine β/γ-lyase from Lactococcus lactis ssp. cremoris MG1363 |
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