Identification and characterization of a cystathionine β/γ-lyase from Lactococcus lactis ssp. cremoris MG1363

This paper describes the nucleotide sequence of a gene encoding cystathionine β/γ-lyase from Lactococcus lactis ssp. cremoris MG1363, its overexpression in Escherichia coli and some functional characteristics of the purified recombinant protein.

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Veröffentlicht in:	FEMS microbiology letters 2000-01, Vol.182 (2), p.249-254
Hauptverfasser:	Dobric, Nada, Limsowtin, Gäetan K.Y., Hillier, Alan J., Dudman, Nicholas P.B., Davidson, Barrie E.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acid derivatization Amino Acid Sequence Bacteriology Biological and medical sciences Cheese - microbiology Cheese flavor Chromatography, Affinity Chromatography, High Pressure Liquid cystathionine Cystathionine gamma-Lyase - chemistry Cystathionine gamma-Lyase - genetics Cystathionine gamma-Lyase - isolation & purification Cystathionine gamma-Lyase - metabolism Cystathionine lyase Cysteine - metabolism Escherichia coli functional properties Fundamental and applied biological sciences. Psychology gene overexpression Genes, Bacterial Homocysteine - metabolism Lactic acid bacterium Lactococcus lactis - enzymology Lactococcus lactis - genetics Lactococcus lactis subsp. cremoris Metabolism. Enzymes Microbiology Molecular Sequence Data nucleotide sequences recombinant proteins Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Alignment Sequence Analysis, DNA Substrate Specificity
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Beschreibung
Zusammenfassung:	This paper describes the nucleotide sequence of a gene encoding cystathionine β/γ-lyase from Lactococcus lactis ssp. cremoris MG1363, its overexpression in Escherichia coli and some functional characteristics of the purified recombinant protein.
ISSN:	0378-1097 1574-6968
DOI:	10.1016/S0378-1097(99)00599-6