From Small-Molecule Reactions to Protein Folding: Studying Biochemical Kinetics by Stopped-Flow Electrospray Mass Spectrometry

This work introduces stopped-flow electrospray ionization (ESI) mass spectrometry (MS) as a method for studying fast biochemical reaction kinetics. After initiating a reaction by rapid mixing of two solutions, the mixture is transferred to a reaction vessel and a steady liquid flow to the ESI source...

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Veröffentlicht in:	Analytical biochemistry 2001-05, Vol.292 (1), p.107-114
Hauptverfasser:	Kolakowski, Beata M, Konermann, Lars
Format:	Artikel
Sprache:	eng
Schlagworte:	chlorophyll Chlorophyll - chemistry demetallation heme Kinetics myoglobin Myoglobin - chemistry Protein Denaturation Protein Folding rapid mixing reaction intermediate Spectrometry, Mass, Electrospray Ionization - methods Time Factors time resolution
Online-Zugang:	Volltext
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