Mesoporous Silica Nanoparticles Improve Magnetic Labeling Efficiency in Human Stem Cells

Tumblerlike magnetic/fluorescein isothiocyanate (FITC)‐labeled mesoporous silica nanoparticles, Mag‐Dye@MSNs, have been developed, which are composed of silica‐coated core–shell superparamagnetic iron oxide (SPIO@SiO2) nanoparticles co‐condensed with FITC‐incorporated mesoporous silica. Mag‐Dye@MSNs can label human mesenchymal stem cells (hMSCs) through endocytosis efficiently for magnetic resonance imaging (MRI) in vitro and in vivo, as manifested by using a clinical 1.5‐T MRI system with requirements of simultaneous low incubation dosage of iron, low detection cell numbers, and short incubation time. Labeled hMSCs are unaffected in their viability, proliferation, and differentiation capacities into adipocytes and osteocytes, which can still be readily detected by MRI. Moreover, a higher MRI signal intensity decrease is observed in Mag‐Dye@MSN‐treated cells than in SPIO@SiO2‐treated cells. This is the first report that MCM‐41‐type MSNs are advantageous to cellular uptake, as manifested by a higher labeling efficiency of Mag‐Dye@MSNs than SPIO@SiO2. Quick on the uptake: Magnetic/fluorescein isothiocyanate (FITC)‐labeled mesoporous silica nanoparticles, Mag‐Dye@MSNs (see picture), capable of efficiently labeling human mesenchymal stem cells, are developed for magnetic resonance imaging. Mag‐Dye@MSNs consist of silica‐coated core/shell superparamagnetic iron oxide (SPIO@SiO2) nanoparticles co‐condensed with FITC‐incorporated mesoporous silica.