A legume lectin from the bark of Robinia pseudoacacia crystallizes in two crystal forms: preliminary diffraction analyses

A legume lectin from the bark of Robinia pseudoacacia crystallizes in two crystal forms: preliminary diffraction analyses

The A4 isoform of the bark lectin RPbAI from Robinia pseudoacacia has been crystallized in two different crystal forms. Crystal form I grows in the P21 space group with two tetramers in the asymmetric unit, whereas crystal form II grows in the I222 space group with a monomer in the asymmetric unit....

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Veröffentlicht in:Acta crystallographica. Section D, Biological crystallography. Biological crystallography., 2000-12, Vol.56 (12), p.1638-1640
Hauptverfasser: Rabijns, A., Verboven, C., Van Damme, E. J. M., Peumans, W. J., De Ranter, C. J.
Format: Artikel
Sprache:eng
Schlagworte:
Crystallization
Fabaceae - chemistry
Lectins - chemistry
Lectins - isolation & purification
legume lectins
Plant Lectins
Plants, Medicinal
Polymers - chemistry
Protein Conformation
R. pseudoacacia
X-Ray Diffraction
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Zusammenfassung:The A4 isoform of the bark lectin RPbAI from Robinia pseudoacacia has been crystallized in two different crystal forms. Crystal form I grows in the P21 space group with two tetramers in the asymmetric unit, whereas crystal form II grows in the I222 space group with a monomer in the asymmetric unit. Data sets were collected for both crystal forms to resolution limits of 2.55 and 1.81 Å, respectively, which will allow successful structure determinations.
ISSN:1399-0047
0907-4449
1399-0047
DOI:10.1107/S0907444900012208