Fabrication of Colloidal Crystal Beads by a Drop-Breaking Technique and Their Application as Bioassays

Fabrication of Colloidal Crystal Beads by a Drop-Breaking Technique and Their Application as Bioassays

Colored beads: Monodisperse colloidal crystal beads with structural colors are used for multiplex immunoassay as encoded biomolecular supports. Mouse, rabbit, and human immunoglobulin G (IgG) are immobilized on blue, green, and red beads, respectively, and incubated with the analyte. The fluorescenc...

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Veröffentlicht in:Small (Weinheim an der Bergstrasse, Germany) Germany), 2008-05, Vol.4 (5), p.592-596
Hauptverfasser: Sun, Cheng, Zhao, Xiang-Wei, Zhao, Yuan-Jin, Zhu, Rong, Gu, Zhong-Ze
Format: Artikel
Sprache:eng
Schlagworte:
bioassays
Biological Assay - methods
colloids
Colloids - chemistry
crystal growth
Crystallization - methods
Macromolecular Substances - chemistry
Materials Testing
microfluidics
Microfluidics - methods
Molecular Conformation
Nanostructures - chemistry
Nanostructures - ultrastructure
Nanotechnology - methods
Particle Size
Polytetrafluoroethylene - chemistry
self-assembly
Surface Properties
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Beschreibung
Zusammenfassung:Colored beads: Monodisperse colloidal crystal beads with structural colors are used for multiplex immunoassay as encoded biomolecular supports. Mouse, rabbit, and human immunoglobulin G (IgG) are immobilized on blue, green, and red beads, respectively, and incubated with the analyte. The fluorescence peaks for the blue and green beads imply that the analyte contains tagged antimouse IgG and antirabbit IgG only (see picture).
ISSN:1613-6810
1613-6829
DOI:10.1002/smll.200701026