Increased Paramagnetic Effect of a Lanthanide Protein Probe by Two-Point Attachment

Increased Paramagnetic Effect of a Lanthanide Protein Probe by Two-Point Attachment

A lanthanide ligand is reported, amenable to be immobilized on a protein surface via cysteine residues. This probe yields large single shifts of the protein nuclei resonances in HSQC NMR spectra and causes significant alignment of the protein in the magnetic field, providing additional structural re...

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Veröffentlicht in:Journal of the American Chemical Society 2007-08, Vol.129 (30), p.9292-9293
Hauptverfasser: Keizers, Peter H. J, Desreux, Jean F, Overhand, Mark, Ubbink, Marcellus
Format: Artikel
Sprache:eng
Schlagworte:
Electromagnetic Phenomena
Lanthanoid Series Elements - chemistry
Molecular Probes - chemistry
Nuclear Magnetic Resonance, Biomolecular - methods
Proteins - chemistry
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Zusammenfassung:A lanthanide ligand is reported, amenable to be immobilized on a protein surface via cysteine residues. This probe yields large single shifts of the protein nuclei resonances in HSQC NMR spectra and causes significant alignment of the protein in the magnetic field, providing additional structural restraints. Anchoring the probe by two cysteine residues instead of one is highly beneficial for the size of its effects.
ISSN:0002-7863
1520-5126
DOI:10.1021/ja0725201