Heterogeneous Expression of Tumor-associated Genes in Disseminated Breast Cancer Cells

Heterogeneous Expression of Tumor-associated Genes in Disseminated Breast Cancer Cells

Background: Gene expression profiles were determined to demonstrate heterogeneity of viable disseminated tumor cells (DTC) in the blood of breast cancer patients. Patients and Methods: All patients (n=48) suffered from metastatic disease (M1) and were treated with chemotherapy and/or Herceptin®, re...

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Veröffentlicht in:Anticancer research 2007-07, Vol.27 (4A), p.1769-1776
Hauptverfasser: ZIEGLSCHMID, Veit, HOLLMANN, Christiane, GUTIERREZ, Bertha, KREHAN, Andreas, KAUL, Sepp, BOCHER, Oliver
Format: Artikel
Sprache:eng
Schlagworte:
Antigens, Neoplasm - genetics
Antigens, Neoplasm - metabolism
Biological and medical sciences
Breast Neoplasms - genetics
Breast Neoplasms - pathology
Cell Adhesion Molecules - genetics
Cell Adhesion Molecules - metabolism
Claudins
Epithelial Cell Adhesion Molecule
Female
Gene Expression
Gene Expression Profiling
Genes, erbB-2
Gynecology. Andrology. Obstetrics
Humans
Immunomagnetic Separation - methods
Mammary gland diseases
Medical sciences
Membrane Proteins - genetics
Membrane Proteins - metabolism
Middle Aged
Mucin-1 - genetics
Mucin-1 - metabolism
Neoplasm Metastasis - genetics
Neoplastic Cells, Circulating - metabolism
Reverse Transcriptase Polymerase Chain Reaction
RNA, Messenger - analysis
Sensitivity and Specificity
Tumors
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Zusammenfassung:Background: Gene expression profiles were determined to demonstrate heterogeneity of viable disseminated tumor cells (DTC) in the blood of breast cancer patients. Patients and Methods: All patients (n=48) suffered from metastatic disease (M1) and were treated with chemotherapy and/or Herceptin®, respectively. Blood samples were analyzed by a DTC detection assay consisting of immunomagnetic tumor cell selection combined with expression profiling of the tumor-associated transcripts GA733-2, MUC-1, HER-2 and Claudin-7. In addition, the correlation of HER-2 expression in DTC with histopathologically determined HER-2 status in distant metastases and primary tumors in selected cases was investigated. Results: DTC were detected in 69% (p
ISSN:0250-7005
1791-7530