Drug Assessment Based on Detection of l-Glutamate Released from C6 Glioma Cells Using an Enzyme−Luminescence Method

Monitoring of excitation activity of nerve cells is very useful for not only brain research but also assessment of the effects of various chemicals, including drugs and toxins. We previously reported a novel enzyme−luminescence method for real-time monitoring of l-glutamate release from C6 glioma ce...

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Veröffentlicht in:	Analytical chemistry (Washington) 2008-05, Vol.80 (10), p.3762-3768
Hauptverfasser:	Zakir Hossain, S. M, Shinohara, Hiroaki, Kitano, Hiromi
Format:	Artikel
Sprache:	eng
Schlagworte:	4-Aminopyridine - administration & dosage Analytical chemistry Animals Brain Neoplasms - enzymology Brain Neoplasms - metabolism Brain Neoplasms - pathology Brain research Carbamazepine - administration & dosage Cell Line, Tumor Cells Chemistry Drugs Enzymes Enzymes - metabolism Exact sciences and technology Flunarizine - administration & dosage Glioma - enzymology Glioma - metabolism Glioma - pathology Glutamic Acid - metabolism Lidocaine - administration & dosage Luminescence Nickel - administration & dosage Nifedipine - administration & dosage Pharmacology Rats Spectrometric and optical methods Veratridine - administration & dosage
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Zusammenfassung:	Monitoring of excitation activity of nerve cells is very useful for not only brain research but also assessment of the effects of various chemicals, including drugs and toxins. We previously reported a novel enzyme−luminescence method for real-time monitoring of l-glutamate release from C6 glioma cells with high levels of sensitivity (≈10 nM) and temporal resolution (
ISSN:	0003-2700 1520-6882
DOI:	10.1021/ac702392p