Molecular epidemiology of Pseudomonas aeruginosa in adult patients with cystic fibrosis in Northern Ireland

Isolates (n=51) of Pseudomonas aeruginosa obtained from the sputa of 29 adult patients attending the Regional Cystic Fibrosis Centre in Northern Ireland were compared using an enterobacterial repetitive intergenic consensus sequence (ERIC2) primer in a random amplification of polymorphic DNA (RAPD)...

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Veröffentlicht in:	British journal of biomedical science 2008-01, Vol.65 (1), p.18-21
Hauptverfasser:	Clarke, L., Moore, J.E., Millar, B.C., Crowe, M., Xu, J., Goldsmith, C.E., Murphy, P.G., Dooley, J.S.G., Rendall, J., Elborn, J.S.
Format:	Artikel
Sprache:	eng
Schlagworte:	Adolescent Adult Aged Bacteria Bacteriology Cystic fibrosis Cystic Fibrosis - epidemiology Cystic Fibrosis - microbiology DNA polymerase DNA Primers DNA, Bacterial - genetics Environmental conditions Female Genotype Genotyping Humans Male Microbiology Molecular Epidemiology Mortality Northern Ireland - epidemiology Patients Pseudomonas aeruginosa Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa - isolation & purification Pseudomonas Infections - epidemiology Pseudomonas Infections - microbiology Random Amplified Polymorphic DNA Technique - methods Random Amplified Polymorphic DNA Technique - standards Recurrence Research methodology Sputum - chemistry Thermal cycling
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Zusammenfassung:	Isolates (n=51) of Pseudomonas aeruginosa obtained from the sputa of 29 adult patients attending the Regional Cystic Fibrosis Centre in Northern Ireland were compared using an enterobacterial repetitive intergenic consensus sequence (ERIC2) primer in a random amplification of polymorphic DNA (RAPD) polymerase chain reaction (PCR) method. Resulting banding patterns showed a high degree of genetic heterogeneity among all isolates from the patients examined, suggesting a non-clonal relationship between isolates from these patients, when employing this genotyping technique.
ISSN:	0967-4845 2474-0896
DOI:	10.1080/09674845.2008.11732789