Mapping of genes expressed in activated porcine Peyer's patch

To determine the chromosomal locations for genes expressed in porcine Peyer's patches, polymerase chain reaction-based mapping of expressed sequence tags (ESTs) isolated from a porcine Peyer's patch-specific cDNA library was performed across a 6500-rad swine radiation hybrid panel. A total...

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Veröffentlicht in:	Animal genetics 2006-02, Vol.37 (1), p.62-65
Hauptverfasser:	Dvorak, C.M.T, Flickinger, G.H, Hendrickson, J.A, Fahrenkrug, S.C, Murtaugh, M.P, Rutherford, M.S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals comparative mapping complementary DNA Expressed Sequence Tags gene expression Gene Library genes Genes - genetics genomic libraries Lod Score mucosal immunity mucosal immunology Peyer's patch Peyer's patches Peyer's Patches - metabolism physical chromosome mapping polymerase chain reaction Radiation Hybrid Mapping sequence alignment Sus scrofa - genetics Sus scrofa - metabolism swine
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Beschreibung
Zusammenfassung:	To determine the chromosomal locations for genes expressed in porcine Peyer's patches, polymerase chain reaction-based mapping of expressed sequence tags (ESTs) isolated from a porcine Peyer's patch-specific cDNA library was performed across a 6500-rad swine radiation hybrid panel. A total of 116 ESTs were mapped with LOD scores >6.0, and another 11 ESTs had LOD scores between 5.0 and 6.0. Of these 127 ESTs, 63% matched known genes (
ISSN:	0268-9146 1365-2052
DOI:	10.1111/j.1365-2052.2005.01377.x