Comparative Proteomic Expression Profile in All-trans Retinoic Acid Differentiated Neuroblastoma Cell Line
Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid t...
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| Veröffentlicht in: | Journal of proteome research 2007-07, Vol.6 (7), p.2550-2564 |
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| container_issue | 7 |
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| container_title | Journal of proteome research |
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| creator | Cimmino, Flora Spano, Daniela Capasso, Mario Zambrano, Nicola Russo, Roberta Zollo, Massimo Iolascon, Achille |
| description | Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which Gβ2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer. Keywords: neuroblastoma • all-trans-retinoic acid • 2D-DIGE • LAN-5 human neuroblastoma cell line |
| doi_str_mv | 10.1021/pr060701g |
| format | Article |
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We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which Gβ2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer. 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| ispartof | Journal of proteome research, 2007-07, Vol.6 (7), p.2550-2564 |
| issn | 1535-3893 1535-3907 |
| language | eng |
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| source | MEDLINE; American Chemical Society Journals |
| subjects | Cell Line, Tumor Cell Nucleus - chemistry Chromatography, Liquid Computational Biology Cytosol - chemistry Electrophoresis, Gel, Two-Dimensional Humans Neoplasm Proteins - analysis Neuroblastoma - chemistry Neuroblastoma - metabolism Peripheral Nervous System Neoplasms - chemistry Peripheral Nervous System Neoplasms - metabolism Protein Array Analysis Proteomics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tretinoin - pharmacology |
| title | Comparative Proteomic Expression Profile in All-trans Retinoic Acid Differentiated Neuroblastoma Cell Line |
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