Comparative Proteomic Expression Profile in All-trans Retinoic Acid Differentiated Neuroblastoma Cell Line

Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid t...

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Veröffentlicht in:	Journal of proteome research 2007-07, Vol.6 (7), p.2550-2564
Hauptverfasser:	Cimmino, Flora, Spano, Daniela, Capasso, Mario, Zambrano, Nicola, Russo, Roberta, Zollo, Massimo, Iolascon, Achille
Format:	Artikel
Sprache:	eng
Schlagworte:	Cell Line, Tumor Cell Nucleus - chemistry Chromatography, Liquid Computational Biology Cytosol - chemistry Electrophoresis, Gel, Two-Dimensional Humans Neoplasm Proteins - analysis Neuroblastoma - chemistry Neuroblastoma - metabolism Peripheral Nervous System Neoplasms - chemistry Peripheral Nervous System Neoplasms - metabolism Protein Array Analysis Proteomics Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Tretinoin - pharmacology
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Zusammenfassung:	Neuroblastoma (NB) is an infant tumor which frequently differentiates into neurons. We used two-dimensional differential in-gel electrophoresis (2D-DIGE) to analyze the cytosolic and nuclear protein expression patterns of LAN-5 cells following neuronal differentiating agent all-trans-retinoic acid treatment. We identified several candidate proteins, from which Gβ2 and Prefoldin 3 may have a role on NB development. These results strength the use of proteomics to discover new putative protein targets in cancer. Keywords: neuroblastoma • all-trans-retinoic acid • 2D-DIGE • LAN-5 human neuroblastoma cell line
ISSN:	1535-3893 1535-3907
DOI:	10.1021/pr060701g