Lupane-Type Saponins from Leaves of Acanthopanax sessiliflorus and Their Inhibitory Activity on Pancreatic Lipase

Three known saponins, chiisanoside, 11-deoxyisochiisanoside, and isochiisanoside, and one novel saponin, 3,4-seco-4(23),20(29)-lupadiene-3,28-dioic acid 28-O-α-l-rhamnopyranosyl (1→4)-β-d-glucopyranosyl (1→6)-β-d-glucopyranoside, referred to as sessiloside, were isolated from a hot water extract of Acanthopanax sessiliflorus leaves. All of these saponins were lupane-type triterpene triglycosides, and their concentrations were 4.1, 1.0, 0.5, and 0.4% (w/w) of the total extract, respectively. Sessiloside and chiisanoside inhibited pancreatic lipase activity in vitro, and addition of the saponin-rich fraction to a high-fat diet suppressed the body weight gain of mice. The possibility of application of the lupane-type saponins from A. sessiliflorus leaves to the treatment of obesity is discussed. Keywords: Lipase inhibitor; Acanthopanax sessiliflorus; lupane-type saponin; chiisanoside; sessiloside; obesity