Chromogenic and Fluorogenic Assays for the Lactonase Activity of Serum Paraoxonases

5‐Thioalkyl butyrolactones (1) are shown to be useful chromogenic/fluorogenic probes for determining lactonase activities, in particular of serum paraoxonase (PON1). These novel probes are suitable for measuring lactonase activity in complex biological samples, including sera, intact cells and cell...

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Veröffentlicht in:	Chembiochem : a European journal of chemical biology 2006-01, Vol.7 (1), p.49-53
Hauptverfasser:	Khersonsky, Olga, Tawfik, Dan S
Format:	Artikel
Sprache:	eng
Schlagworte:	Aryldialkylphosphatase - blood Aryldialkylphosphatase - chemistry Carboxylic Ester Hydrolases - blood Carboxylic Ester Hydrolases - chemistry Chromogenic Compounds - chemistry enzyme fingerprinting Enzyme-Linked Immunosorbent Assay - methods Flow Cytometry - methods Fluorescent Dyes - chemistry fluorescent probes Humans Hydrogen-Ion Concentration Hydrolysis lactones Lactones - chemical synthesis Lactones - chemistry Molecular Structure phenotyping PON1 Time Factors
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Beschreibung
Zusammenfassung:	5‐Thioalkyl butyrolactones (1) are shown to be useful chromogenic/fluorogenic probes for determining lactonase activities, in particular of serum paraoxonase (PON1). These novel probes are suitable for measuring lactonase activity in complex biological samples, including sera, intact cells and cell lysates.
ISSN:	1439-4227 1439-7633
DOI:	10.1002/cbic.200500334