LC-ESI-MSD fast determination of residual mitomycin C in hen aqueous humour after corneal refractive surgery

A simple, fast and reliable method has been developed for the assay of traces of mitomycin C (MMC) in hen aqueous humour samples. The determination was carried out by high-performance liquid chromatography with electrospray ionization mass spectrometric detection. In isocratic elution analysis, the mobile phase was a mixture of water–acetonitrile (78:22, v/v) and the chromatographic column was C 18 at 35 °C. The method has been validated over a range from 0.1 to 250 μg L −1 in hen aqueous humour with correlation coefficients higher than 0.999. Limit of detection and limit of quantification for MMC based in signal to noise ratio of 3 and 10, respectively, were 20 and 71 ng L −1. The developed method allows the analysis of MMC in hen aqueous humour samples obtained at different times and conditions in order to evaluate and compare the efficacy of the drug administration.