Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose
To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins we...
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| Veröffentlicht in: | Proteomics (Weinheim) 2007-06, Vol.7 (13), p.2184-2188 |
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| container_issue | 13 |
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| container_title | Proteomics (Weinheim) |
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| creator | Kim, Pan-Kyeom Kim, Mi-Ryung Kim, Hyun-Jung Yoo, Hyun-Syuk Kim, Ji-Soo Cho, Eun-Hee Kim, Chan-Wha |
| description | To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2‐DE and ESI‐Q‐TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose. |
| doi_str_mv | 10.1002/pmic.200700051 |
| format | Article |
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The differentially expressed HSC proteins were analyzed using 2‐DE and ESI‐Q‐TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.</description><identifier>ISSN: 1615-9853</identifier><identifier>EISSN: 1615-9861</identifier><identifier>DOI: 10.1002/pmic.200700051</identifier><identifier>PMID: 17549797</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Actins - metabolism ; Adipocytes - cytology ; Adipocytes - drug effects ; Adipocytes - metabolism ; Analytical, structural and metabolic biochemistry ; Animals ; Biological and medical sciences ; Down-Regulation - drug effects ; Electrophoresis, Gel, Two-Dimensional ; Electrospray-quadrupole-time-of-flight mass spectrometry ; Fundamental and applied biological sciences. Psychology ; Galectins - metabolism ; Glucose - pharmacology ; Glycolysis - drug effects ; Hepatic stellate cell ; High glucose ; Liver - cytology ; Miscellaneous ; Peroxidases - metabolism ; Peroxiredoxins ; Proteins ; Proteins - analysis ; Proteins - metabolism ; Proteome - analysis ; Proteome - drug effects ; Proteome - metabolism ; Rats ; Spectrometry, Mass, Electrospray Ionization ; Tandem Mass Spectrometry ; Triose-Phosphate Isomerase - metabolism ; Two-dimensional gel electrophoresis ; Up-Regulation - drug effects</subject><ispartof>Proteomics (Weinheim), 2007-06, Vol.7 (13), p.2184-2188</ispartof><rights>Copyright © 2007 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><rights>2007 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3261-6982ad8798dedb3aaf633032cb9ac2e39fa4fbf75fccd17da52f0549bd92e6883</citedby><cites>FETCH-LOGICAL-c3261-6982ad8798dedb3aaf633032cb9ac2e39fa4fbf75fccd17da52f0549bd92e6883</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fpmic.200700051$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fpmic.200700051$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=18891816$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17549797$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, Pan-Kyeom</creatorcontrib><creatorcontrib>Kim, Mi-Ryung</creatorcontrib><creatorcontrib>Kim, Hyun-Jung</creatorcontrib><creatorcontrib>Yoo, Hyun-Syuk</creatorcontrib><creatorcontrib>Kim, Ji-Soo</creatorcontrib><creatorcontrib>Cho, Eun-Hee</creatorcontrib><creatorcontrib>Kim, Chan-Wha</creatorcontrib><title>Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose</title><title>Proteomics (Weinheim)</title><addtitle>Proteomics</addtitle><description>To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2‐DE and ESI‐Q‐TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.</description><subject>Actins - metabolism</subject><subject>Adipocytes - cytology</subject><subject>Adipocytes - drug effects</subject><subject>Adipocytes - metabolism</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Down-Regulation - drug effects</subject><subject>Electrophoresis, Gel, Two-Dimensional</subject><subject>Electrospray-quadrupole-time-of-flight mass spectrometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Galectins - metabolism</subject><subject>Glucose - pharmacology</subject><subject>Glycolysis - drug effects</subject><subject>Hepatic stellate cell</subject><subject>High glucose</subject><subject>Liver - cytology</subject><subject>Miscellaneous</subject><subject>Peroxidases - metabolism</subject><subject>Peroxiredoxins</subject><subject>Proteins</subject><subject>Proteins - analysis</subject><subject>Proteins - metabolism</subject><subject>Proteome - analysis</subject><subject>Proteome - drug effects</subject><subject>Proteome - metabolism</subject><subject>Rats</subject><subject>Spectrometry, Mass, Electrospray Ionization</subject><subject>Tandem Mass Spectrometry</subject><subject>Triose-Phosphate Isomerase - metabolism</subject><subject>Two-dimensional gel electrophoresis</subject><subject>Up-Regulation - drug effects</subject><issn>1615-9853</issn><issn>1615-9861</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS1U1JbSK0fkS3vLYscbfxzLqiyVCrQSCG7WxBl3TZN4ayeC_e_JsqstN05vDr838_SGkDeczThj5bt1F9ysZEwxxir-gpxyyavCaMmPDnMlTsirnH8yxpU26piccFXNjTLqlPy4S3HA2CGFHtpNDplGT4cV0gQDXeEahuBoHrBtYUDqJs107BtMdBUeVtTF3mE_THCI_V_vQzu6mPE1eemhzXi-1zPy7cP118XH4vbL8mZxdVs4UUpeSKNLaLQyusGmFgBeCsFE6WoDrkRhPMx97VXlnWu4aqAqPZvC140pUWotzsjlbu86xacR82C7kLcxocc4ZquYVGqu5ATOdqBLMeeE3q5T6CBtLGd226XddmkPXU6Gt_vNY91h84zvy5uAiz0A2UHrE_Qu5GdOa8M13142O-5XaHHzn7P27tPN4t8Qxc4bph_8PnghPVqphKrs989Le3-_eF-pJbdL8QcUw55N</recordid><startdate>200706</startdate><enddate>200706</enddate><creator>Kim, Pan-Kyeom</creator><creator>Kim, Mi-Ryung</creator><creator>Kim, Hyun-Jung</creator><creator>Yoo, Hyun-Syuk</creator><creator>Kim, Ji-Soo</creator><creator>Cho, Eun-Hee</creator><creator>Kim, Chan-Wha</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><general>Wiley-VCH</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200706</creationdate><title>Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose</title><author>Kim, Pan-Kyeom ; Kim, Mi-Ryung ; Kim, Hyun-Jung ; Yoo, Hyun-Syuk ; Kim, Ji-Soo ; Cho, Eun-Hee ; Kim, Chan-Wha</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3261-6982ad8798dedb3aaf633032cb9ac2e39fa4fbf75fccd17da52f0549bd92e6883</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Actins - metabolism</topic><topic>Adipocytes - cytology</topic><topic>Adipocytes - drug effects</topic><topic>Adipocytes - metabolism</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Down-Regulation - drug effects</topic><topic>Electrophoresis, Gel, Two-Dimensional</topic><topic>Electrospray-quadrupole-time-of-flight mass spectrometry</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Galectins - metabolism</topic><topic>Glucose - pharmacology</topic><topic>Glycolysis - drug effects</topic><topic>Hepatic stellate cell</topic><topic>High glucose</topic><topic>Liver - cytology</topic><topic>Miscellaneous</topic><topic>Peroxidases - metabolism</topic><topic>Peroxiredoxins</topic><topic>Proteins</topic><topic>Proteins - analysis</topic><topic>Proteins - metabolism</topic><topic>Proteome - analysis</topic><topic>Proteome - drug effects</topic><topic>Proteome - metabolism</topic><topic>Rats</topic><topic>Spectrometry, Mass, Electrospray Ionization</topic><topic>Tandem Mass Spectrometry</topic><topic>Triose-Phosphate Isomerase - metabolism</topic><topic>Two-dimensional gel electrophoresis</topic><topic>Up-Regulation - drug effects</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Pan-Kyeom</creatorcontrib><creatorcontrib>Kim, Mi-Ryung</creatorcontrib><creatorcontrib>Kim, Hyun-Jung</creatorcontrib><creatorcontrib>Yoo, Hyun-Syuk</creatorcontrib><creatorcontrib>Kim, Ji-Soo</creatorcontrib><creatorcontrib>Cho, Eun-Hee</creatorcontrib><creatorcontrib>Kim, Chan-Wha</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Proteomics (Weinheim)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Pan-Kyeom</au><au>Kim, Mi-Ryung</au><au>Kim, Hyun-Jung</au><au>Yoo, Hyun-Syuk</au><au>Kim, Ji-Soo</au><au>Cho, Eun-Hee</au><au>Kim, Chan-Wha</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose</atitle><jtitle>Proteomics (Weinheim)</jtitle><addtitle>Proteomics</addtitle><date>2007-06</date><risdate>2007</risdate><volume>7</volume><issue>13</issue><spage>2184</spage><epage>2188</epage><pages>2184-2188</pages><issn>1615-9853</issn><eissn>1615-9861</eissn><abstract>To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2‐DE and ESI‐Q‐TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>17549797</pmid><doi>10.1002/pmic.200700051</doi><tpages>5</tpages></addata></record> |
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| subjects | Actins - metabolism Adipocytes - cytology Adipocytes - drug effects Adipocytes - metabolism Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Down-Regulation - drug effects Electrophoresis, Gel, Two-Dimensional Electrospray-quadrupole-time-of-flight mass spectrometry Fundamental and applied biological sciences. Psychology Galectins - metabolism Glucose - pharmacology Glycolysis - drug effects Hepatic stellate cell High glucose Liver - cytology Miscellaneous Peroxidases - metabolism Peroxiredoxins Proteins Proteins - analysis Proteins - metabolism Proteome - analysis Proteome - drug effects Proteome - metabolism Rats Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Triose-Phosphate Isomerase - metabolism Two-dimensional gel electrophoresis Up-Regulation - drug effects |
| title | Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose |
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