Proteome analysis of the rat hepatic stellate cells under high concentrations of glucose

To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins we...

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Veröffentlicht in:	Proteomics (Weinheim) 2007-06, Vol.7 (13), p.2184-2188
Hauptverfasser:	Kim, Pan-Kyeom, Kim, Mi-Ryung, Kim, Hyun-Jung, Yoo, Hyun-Syuk, Kim, Ji-Soo, Cho, Eun-Hee, Kim, Chan-Wha
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins - metabolism Adipocytes - cytology Adipocytes - drug effects Adipocytes - metabolism Analytical, structural and metabolic biochemistry Animals Biological and medical sciences Down-Regulation - drug effects Electrophoresis, Gel, Two-Dimensional Electrospray-quadrupole-time-of-flight mass spectrometry Fundamental and applied biological sciences. Psychology Galectins - metabolism Glucose - pharmacology Glycolysis - drug effects Hepatic stellate cell High glucose Liver - cytology Miscellaneous Peroxidases - metabolism Peroxiredoxins Proteins Proteins - analysis Proteins - metabolism Proteome - analysis Proteome - drug effects Proteome - metabolism Rats Spectrometry, Mass, Electrospray Ionization Tandem Mass Spectrometry Triose-Phosphate Isomerase - metabolism Two-dimensional gel electrophoresis Up-Regulation - drug effects
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Zusammenfassung:	To study the change in hepatic stellate cell (HSC) function under diabetic conditions, we cultured rat HSC in the presence of 5 and 30 mM glucose, which correspond to blood glucose concentrations during the early and late stages of diabetes, respectively. The differentially expressed HSC proteins were analyzed using 2‐DE and ESI‐Q‐TOF MS/MS and confirmed with Western blotting. The changed protein expression will provide greater understanding of glycolysis in HSC at the high concentration of glucose.
ISSN:	1615-9853 1615-9861
DOI:	10.1002/pmic.200700051