The HLA-DQB alleles and amino acid variants of the vitamin D-binding protein in diabetic patients in Alsace
Background: The HLA-DQB1 chain, in particular the amino acid in position 57, and genetic variants of the vitamin D-binding protein (DBP) have been reported to be associated with type 1 diabetes. There are two known polymorphisms in exon 11 of the DBP gene resulting in amino acid variants: codons 416...
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Veröffentlicht in: | Clinical biochemistry 2001-02, Vol.34 (1), p.59-63 |
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Zusammenfassung: | Background: The HLA-DQB1 chain, in particular the amino acid in position 57, and genetic variants of the vitamin D-binding protein (DBP) have been reported to be associated with type 1 diabetes. There are two known polymorphisms in exon 11 of the DBP gene resulting in amino acid variants: codons 416 GA
T → GA
G (Asp → Glu) and 420 A
CG → A
AG (Thr → Lys). We compared distribution of DQB1 alleles and amino acid variants of DBP in type 1 diabetic patients (
n = 44) in the Alsacian population and in healthy controls (
n = 58).
Methods: The second exon of the DQB1 gene and exon 11 of DBP were analyzed by restriction mapping after polymerase chain reaction.
Results: A significant enrichment in DQB1 alleles encoding for an amino acid different from Asp in position 57 (NA) was observed in diabetic subjects as compared to controls (94.3 vs. 32.8%;
p < 0.001). Combinations other than Ala/Ala carried the highest relative risk (OR = 52;
p < 0.001). The analysis of the polymorphism in exon 11 of DBP showed a significant difference in the allele frequency of the HaeIII site, but not of the StyI site between patients and controls. Allele frequencies of HaeIII in diabetic subjects were 36% and 64% for Asp and Glu respectively (
p < 0.001; χ
2 = 29.5). The frequency of Asp/Asp and Glu/Glu genotypes was increased in controls and diabetic subjects respectively. DBP alleles in individuals carrying the DQB1 NA combination revealed that 46.6% of diabetics were DBP Asp/Glu, but this was not statistically significant using the Fisher exact test (16/31 vs. 0/3;
p = 0.23).
Conclusions: The study of the DQB1 chain confirmed the value of alleles encoding for an amino acid different from Asp in position 57 (NA) in the susceptibility to type 1 diabetes. The allele frequency of the HaeIII site, but not of the StyI site, differed between patients and controls (HaeIII
p < 0.001; StyI
p > 0.05). |
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ISSN: | 0009-9120 1873-2933 |
DOI: | 10.1016/S0009-9120(00)00197-1 |