Identification and characterization of diarrheal microorganisms

Acute diarrhea belongs to group of diseases caused by pathogenic microorganisms - Salmonella, Shigella and pathogenic strains of E.coli. Despite the prophylactic measurements morbidity rate of diarrheal diseases remains very high especially in children and in traveling persons. Among the main causes of illness is emerging of new strains of microorganisms, new serological versions of already known pathogens or antibiotic resistant strains of these pathogens. Struggle against the resistant forms of microorganism with help of antibiotics and a chemical preparation is becoming less effective. Therefore the problem for searching alternative remedies against above mentioned bacteria with help of viruses - bacteriophages is set. Various selective media and differentiating media absorbed and specific sera were used to identify microorganisms. Detailed bacteriological analyses of 159 newly isolated and museum microorganisms, according to standard schemes including plating on selective and differential media, microscopy, and gram stain, determination of biochemical activity and fermentative features, agglutination reaction with different sera have been carried out. Species and genus designation of strains has been determined.