Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates

Protective responses against experimental Mycobacterium leprae infection in mice induced by recombinant Bacillus Calmette-Guérin over-producing three putative protective antigen candidates

The components of Ag85 (Ag85A, Ag85B, and Ag85C) are putative protective antigen candidates against mycobacterial infection. A recombinant Mycobacterium bovis Bacillus Calmette-Guérin (rBCG) over-producing Ag85A, Ag85B, and MPB51 (rBCG/BA51) was constructed. rBCG/BA51 could secrete these antigens at...

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Veröffentlicht in:Vaccine 2001-02, Vol.19 (15), p.1906-1910
Hauptverfasser: Ohara, Naoya, Matsuoka, Masanori, Nomaguchi, Hiroko, Naito, Mariko, Yamada, Takeshi
Format: Artikel
Sprache:eng
Schlagworte:
Ag85
Ag85 protein
Animals
Antigens, Bacterial - genetics
Bacteriology
Base Sequence
BCG
BCG Vaccine - genetics
BCG Vaccine - immunology
BCG Vaccine - pharmacology
Biological and medical sciences
DNA Primers - genetics
Fundamental and applied biological sciences. Psychology
Leprosy
Leprosy - immunology
Leprosy - prevention & control
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Microbiology
Mycobacterium bovis - genetics
Mycobacterium bovis - immunology
Mycobacterium leprae
Mycobacterium leprae - genetics
Mycobacterium leprae - growth & development
Mycobacterium leprae - immunology
Plasmids - genetics
Recombinant
Spleen - immunology
Vaccine
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Vaccines, Synthetic - genetics
Vaccines, Synthetic - immunology
Vaccines, Synthetic - pharmacology
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Zusammenfassung:The components of Ag85 (Ag85A, Ag85B, and Ag85C) are putative protective antigen candidates against mycobacterial infection. A recombinant Mycobacterium bovis Bacillus Calmette-Guérin (rBCG) over-producing Ag85A, Ag85B, and MPB51 (rBCG/BA51) was constructed. rBCG/BA51 could secrete these antigens at levels more than five times higher than parental BCG. Immunization of C57BL/6 and BALB/c mice with this rBCG reduced the multiplication of Mycobacterium leprae in the foot pads of both strains of mice. The inhibition by rBCG/BA51 was more evident than that by parental BCG.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(00)00439-4