Single molecule level detection of allophycocyanin by surface enhanced resonance Raman scattering

Single molecule level detection of the near-infrared fluorescent protein allophycocyanin (APC) has been achieved using surface enhanced resonance Raman scattering (SERRS). The detection limit using the peak height of the 440 cm(-1) band was 1 x 10(-13) mol l(-1), compared to 2 x 10(-12) mol l(-1) fo...

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Veröffentlicht in:	Analyst (London) 2007-07, Vol.132 (7), p.633-634
Hauptverfasser:	MCGUINNESS, Colin D, MACMILLAN, Alexander M, KAROLIN, Jan, SMITH, W. Ewen, GRAHAM, Duncan, PICKUP, John C, BIRCH, David J. S
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Chemistry Exact sciences and technology Fluorescent Dyes Particle Size Phycocyanin - analysis Spectrometric and optical methods Spectrum Analysis, Raman - instrumentation Spectrum Analysis, Raman - methods
Online-Zugang:	Volltext
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Beschreibung
Zusammenfassung:	Single molecule level detection of the near-infrared fluorescent protein allophycocyanin (APC) has been achieved using surface enhanced resonance Raman scattering (SERRS). The detection limit using the peak height of the 440 cm(-1) band was 1 x 10(-13) mol l(-1), compared to 2 x 10(-12) mol l(-1) for the fluorescence peak at 660 nm.
ISSN:	0003-2654 1364-5528
DOI:	10.1039/b706409h