Bright monomeric red fluorescent protein with an extended fluorescence lifetime

Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore...

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Veröffentlicht in:	Nature methods 2007-07, Vol.4 (7), p.555-557
Hauptverfasser:	Chudakov, Dmitriy M, Merzlyak, Ekaterina M, Goedhart, Joachim, Shcherbo, Dmitry, Bulina, Mariya E, Shcheglov, Aleksandr S, Fradkov, Arkady F, Gaintzeva, Anna, Lukyanov, Konstantin A, Lukyanov, Sergey, Gadella, Theodorus W J
Format:	Artikel
Sprache:	eng
Schlagworte:	Bioinformatics Biological Microscopy Biological Techniques Biomedical and Life Sciences Biomedical Engineering/Biotechnology brief-communication Energy transfer Equipment and supplies Fluorescence Fluorescence microscopy Fluorescence Resonance Energy Transfer - methods HeLa Cells Humans Life Sciences Luminescent Agents - chemistry Luminescent Proteins - analysis Luminescent Proteins - chemistry Luminescent Proteins - genetics Optical properties Proteins Proteins - analysis Proteomics Red Fluorescent Protein Research methodology Resonance Scientific imaging
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Zusammenfassung:	Fluorescent proteins have become extremely popular tools for in vivo imaging and especially for the study of localization, motility and interaction of proteins in living cells. Here we report TagRFP, a monomeric red fluorescent protein, which is characterized by high brightness, complete chromophore maturation, prolonged fluorescence lifetime and high pH-stability. These properties make TagRFP an excellent tag for protein localization studies and fluorescence resonance energy transfer (FRET) applications.
ISSN:	1548-7091 1548-7105
DOI:	10.1038/nmeth1062