Analysis of sequence-dependent curvature in matrix attachment regions

Sequence-dependent DNA conformations of matrix attachment regions (MARs) available in a database were calculated using the wedge model, and compared with randomly chosen genes, promoters, enhancers and transposons. The MARs had a longer bent part and higher angle/helical turn than the other regions....

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Veröffentlicht in:FEBS letters 2001-02, Vol.489 (2), p.166-170
Hauptverfasser: Yamamura, Jun, Nomura, Koji
Format: Artikel
Sprache:eng
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Zusammenfassung:Sequence-dependent DNA conformations of matrix attachment regions (MARs) available in a database were calculated using the wedge model, and compared with randomly chosen genes, promoters, enhancers and transposons. The MARs had a longer bent part and higher angle/helical turn than the other regions. It is known that some MAR sequences have A-tracts that cause DNA bending, and we also found many A-tracts in examined MARs. Furthermore, non-random and clustered distribution of A-tracts shown here gave further evidence of the importance of A-tracts for MAR conformations. These results suggest that DNAs of MARs have a characteristic conformation instead of conserved sequence.
ISSN:0014-5793
1873-3468
DOI:10.1016/S0014-5793(01)02098-1